U6504
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U6504
Sigma
Urea
for electrophoresis
| Synonym: | Carbamide, Carbonyldiamide |
| CAS Number: | 57-13-6 |
| Linear Formula: | NH2CONH2 |
| Molecular Weight: | 60.06 |
| Beilstein Registry Number: | 635724 |
| EC Number: | 200-315-5 |
| MDL number: | MFCD00008022 |
| PubChem Substance ID: | 24900435 |
Description
| Application | Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. |
| A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis. |
Properties
| grade | for electrophoresis |
| total impurities | ≤0.005% Ammonia |
| mp | 132-135 °C(lit.) |
| density | 1.335 g/mL at 25 °C(lit.) |
| cation traces | Fe: ≤1 ppm |
| heavy metals (as Pb): ≤5 ppm | |
| abs. | A |
| A | |
| Gene Information | human ... CA1(759), CA2(760) |
| rat ... Ppm1a(24666) |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 1 |
| RTECS | YR6250000 |
References
| reference | O'Farrell, P.H., High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250, 4007, (1975) Abstract |
| Andrews, A.T. Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications 2nd ed., New York , (1988), 252 | |
| Hochstrasser, D.F., Methods for increasing the resolution of two-dimensional protein electrophoresis. Anal. Biochem. 173, 424, (1988) Abstract | |
| Merck | Merck 13,9935 |
| Beilstein | Beil. 3,IV,94 |
| Fieser | Fieser 1,1262 / Fieser 2,455 |
| Corp MSDS 1 (2), 3578:A / FT-IR 2 (1), 1356:D / FT-IR 1 (1), 799:C / IR-Spectra (2), 420:F / IR-Spectra (3), 474:B / NMR-Reference 2 (1), 670:B / RegBook 1 (1), 939:B / Sax 6, 2713 / Structure Index 1, 146:A:1 |
